DAB Horseradish Peroxidase Chromogenic Kit

Catalog# BWR1068  

Size: 1 Kit

Lot # Check on the product label

Introduction

1. By means of Horseradish Peroxidase (HRP), this DAB Chromogenic Kit can be used for color development in Immunohistochemistry, In situ hybridization, Western, Southern, Northern, EMSA.

2. DAB ( 3, 3 N-Diaminobenzidine Tertrahydrochloride), is the common substrate of HRP. Catalyzed by HRP, DAB can produce the brown precipitate insoluble in water and ethanol. Thus, after DAB color development, end user can use ethanol soluble dye to do the successor staining.

3. This kit can be used for color development of HRP combined cells or tissues in Immunohistochemistry or In situ hybridization, HRP conjugated membrane in Western blot, and endogenic HRP of cells and tissues.

4. In Immunohistochemistry or In situ hybridization, this kit can detect about 100 sections (0.2 ml per section).

Kit Components

Protocol

1. After incubating with HRP conjugated antibody, wash with TBS or PBS thoroughly for 4 times, 5 min each.

2. Prepare chromogenic reagent work solution: Add 1 ml of Chromogenic Reagent B into 50 μl of Chromogenic Reagent A and mix thoroughly. Add this work solution on the sample, and develop for 1-10 min.

3. Wash with distilled water to stop the reaction. If necessary, re-stain with haematoxylin for 1-2 min, and wash with water.

4. Dehydrate, transparent, seal with neutral balata and observe under a microscope.