Peroxidase Conjugated anti-Rabbit IgG SABC Kit

Catalog# BAR1015

Size: 1 kit, RTU

Lot # Check on the product label

Introduction

SABC (StreptAvidin-Biotin Complex) is specially designed for displaying the distribution of antigens in tissues and cells in immunochemistry and other immunodetection analyses. Streptavidin is a 47 KDa protein purified from the bacterium Streptomyces avidinii. Streptavidin has extraordinarily strong affinity to biotin molecules. The dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L, a million times higher than the typical affinity between antigens and their antibodies. Streptavidin has very low non-specific binding to tissues and cells, due to its nearly neutral isoelectric point (IP=6.0~6.5). Therefore, immunohistochemical analyses based on streptavidin-biotin complex has extremely low background. Furthermore, this kit has high sensitivity because each complex it generates has a large number of peroxidase and streptavidin molecules. In brief, SABC offers high specificity, low background and ease-of-use.

Kit Components

Application

Immunohistochemistry, IHC-P

Immunohistochemistry, IHC-Fr

Immunocytochemistry, ICC

Material Required But Not Provided

1. APES or POLY-L-LYSINE.

2. 0.02M PBS (pH 7.2~7.6): 8.5g sodium chloride, 2.8g anhydrous Na2HPO4 and 0.4g anhydrous NaH2PO4 in 1000ml of distilled water. (The weight should be adjusted accordingly if hydrous phosphates are used.)

3. 0.01 M Citrate Buffer: 3g sodium citrate dihydrate (C6H5Na3O7·2H2O) and 0.4g citric acid monohydrate (C6H8O7·H2O) in 1000ml of distilled water.

4. DAB Horseradish Peroxidase Chromogenic Kit (Catalog # BWR1068).

5. 0.1% trypsinase or the compound digest solution.