Monoclonal Anti- Chlortetracycline Antibody

Catalog# BDA1016

Lot # Check on the product label

Size 1mg

Host Mouse

Product Form Liquid

Concentration 7.8 mg/ml (Changed for different lots)

Purification & Buffer

Protein A or G purified and supplied in 0.01 M PBS (pH7.4)

Purity >95% by HPLC & SDS-PAGE

IC50 Value

0.8-1.2 ppb

Sensitivity 0.1 ppb

Recommend Application

ELISA (1:100000)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Aliquot and store at -20°C for 2 years. 

Avoid repeated freeze and thaw cycles.

Background

Chlortetracycline is a tetracycline antibiotic, the first tetracycline to be identified. It was discovered in 1945 by Benjamin Minge Duggar working at Lederle Laboratories under the supervision of Yellapragada Subbarow. Serum  chlortetracycline (CTC) concentration in dromedary camels increases within 1 hour after intrauterine administration and remains elevated for at least 72 hours in control, oxytocin-, and PGF2α-treated animals. Pulicharla R et al investigated the stability constants and toxicity of chlortetracycline (CTC) and metal (Ca, Mg, Cu and Cr) complexes. Their study provides evidence that CTC and its metal complexes are toxic to bacteria when they are biologically available. As for WWS, CTC was adsorbed to solid part and was not biologically available to show measurable toxic effects.

Reference

1. Jukes, Thomas H. Some historical notes on chlortetracycline. Reviews of Infectious Diseases 7(5):702-707 (1985).

2. Ghoneim IM, Abdelghany AM, Waheed MM, Elmoslemany AM, Alhaider AK, Al-Eknah MM. Effect of oxytocin and PGF2α on chlortetracycline absorption from the uterus of early postpartum camels (Camelus dromedarius). Theriogenology. 2015 Sep 1;84(4):645-9.

3. Pulicharla R, Das RK, Brar SK, Drogui P, Sarma SJ, Verma M, Surampalli RY, Valero JR. Toxicity of chlortetracycline and its metal complexes to model microorganisms in wastewater sludge. Sci Total Environ. 2015 Nov 1;532:669-75.