Collagenase IV

Catalog# BBC1004

Size: 100 mg 

Lot # Check on the product label

CAS No .: 9001-12-1

Storage Instruction: Store Collagenase powder at 2-8°C.Reconstituted solution should be stored at -20°C. Avoid repeated freezing and thawing.

Introduction

Collagenase (also called Clostridiopeptidase A) is an enzyme derived from Clostridium histolyticum.Collagenases are endopeptidases that digest native collagen in triple helix region. They show specificity for the sequence R-Pro-(-X-Gly-Pro),whereXis most often a neutral amino acid.Bacterial collagenases have a broader specificity than vertebrate collagenases. Bacterial collagenases can degrade both, water-insoluble native collagens and water-soluble denatured collagens. The extracellular matrix in animaltissue is a complex mixture of collagens and other extracellular matrix proteins like glycoproteins and proteoglycans. This matrix must be effectively broken down to isolate single cells, without alteration of cellular structures. Crude collagenase is a mixture of collagenases and other proteolytic enzymes. It contains a sulphydryl protease, clostripain, a trypsinlike enzyme and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation.