Recombinant ACE2 Protein (Capture)

Catalog# BRP1138

Lot # Check on the product label

Size 1 mg

Description

Recombinant Angiotensin-converting enzyme 2, ACE2 protein with hFc tag.

Synonyms Angiotensin-converting enzyme 2, ACE2

Source HEK293

Purity >95% by SDS-PAGE

Formulation Liquid

Application

ELISA

Capture: BRP1138

Detection: BRP1132

BRP1132 need to be conjugated to HRP or Biotin for ELISA assay.

Other applications have not been tested. 

The optimal dilutions should be determined by end user.

Storage buffer

Each vial contains 0.01 M PBS (pH7.4).

Storage & Expiration

Ship at 4℃. Upon receipt, aliquot and store at -20℃ for long term.

Avoid repeated freeze and thaw cycles.

Background

Angiotensin converting enzyme 2 (ACE-2), also known as ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 also serves as the receptor for the SARS-CoV and SARS-CoV-2 viruses. Binding of the S1 domain of the SARS Coronavirus Spike protein to ACE-2 initiates viral entry into the host cell. Recently, this interaction between SARS-CoV-2 and ACE-2 has become an area of intense therapeutic interest to develop treatments against COVID-19.

Reference

1. Li, W., Moore, M. J., Vasilieva, N., Sui, J., Wong, S. K., Berne, M. A.,... Farzan, M. (2003). Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature, 426(6965), 450-454.

2. Yu YT, Chien SC, Chen IY, Lai CT, Tsay YG, Chang SC, Chang MF. (2016) Surface vimentin is critical for the cell entry of SARS-CoV. J Biomed Sci.