EASYspin RNA Isolation Kit (For the whole blood)
Size: 50 T
Lot # Check on the product label
--------------------------------------------------------------------------------------------------------------
Price: $300.00
Catalog# BPC1007
Size: 50 T
Lot # Check on the product label
Description
The Easyspin RNA Isolation Kit offers a fast and easy way to extract high quality RNA from tissues and cells. The whole process is phenol/chloroform-free. Red blood cell lysate selectively lyses red blood cells, and then the unique lysis buffer/ β-mercaptoethanol immediately lyses biological samples and inactivates RNase and DNase. Ethanol is added to the lysate to provide appropriate binding conditions for RNA, and RNA selectively binds to the silica-membrane of the RNA column in the high-salt buffer. RNA is purified through a series of wash-spin steps to remove protein followed by elution of RNA from silica membrane with RNase-free H2O.
Kit Contents and Storage
|
Components |
Size |
Storage |
|
10X red blood cell lysate RLB |
25 ml |
Store at RT for 9 months.
|
|
Buffer RLT |
50 ml |
|
|
Buffer RW1 |
40 ml |
|
|
Wash Buffer RW |
10 ml |
|
|
RNase-free H2O |
10 ml |
|
|
70% Ethanol |
9 ml RNase free H2O |
|
|
RNA Bind Columns |
50 pcs |
Storage precautions
1. All reagent solutions should be clear. If the ambient temperature is low, the solution may precipitate and should not be used directly. It can be heated in a 37°C water bath and used after it is clear.
