EASYspin RNA Isolation Kit (For fixed / embedded tissues)
Size: 50 T
Lot # Check on the product label
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Price: $370.00
Catalog# BPC1014
Size: 50 T
Lot # Check on the product label
Description
This kit is designed to quickly extract total RNA from formalin-fixed and paraffin-embedded tissue samples. The unique lysis solution/proteinase K quickly lyses cells to release RNA, and then the lysis mixture passes through a genomic DNA removal column, where genomic DNA is removed and RNA is filtered through. After the filtered RNA is adjusted with ethanol to adjust the binding conditions, the RNA is selectively adsorbed on the silicon matrix membrane in the spin column in a high-isolated salt state, and then through a series of rapid rinsing-centrifugation steps, the protein-removing solution and the rinsing solution remove the cells. Metabolites, proteins and other impurities are removed, and finally the low-salt RNase free H20 will elute the pure RNA from the silicon matrix membrane. The obtained RNA can be used in experiments such as reverse transcription PCR and fluorescent quantitative PCR.
Kit Contents and Storage
|
Components |
Size |
Storage |
|
Buffer PKD |
15 ml |
Store at RT for 12 months.
|
|
Buffer RBC |
25 ml |
|
|
Wash Buffer RW |
10 ml |
|
|
Proteinase K powder (40mg/ml) |
20 mg |
Store at -20°C for 12 months. |
|
RNase-free H2O |
10 ml |
Store at RT for 12 months.
|
|
Genomic DNA Elimination Columns |
50 pcs |
|
|
RNase-free Bind Columns |
50 pcs |
