Whole Blood DNA Kit (Large)
Size: 96 T×10 ml
Lot # Check on the product label
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Price: $1380.00
Catalog# BPC1142
Size: 96 T×10 ml
Lot # Check on the product label
Description
This kit uses several rapid steps to extract genomic DNA based on the characteristics of whole blood. First, the erythrocyte lysate is lysed to remove red blood cells without DNA, the nuclear lysate lyses leukocytes to release genomic DNA, and then the protein precipitation solution is selectively precipitated to remove proteins. Finally, the purified genomic DNA is precipitated by isopropanol and redissolved in the DNA lysate.
Kit Contents and Storage
|
Components |
Size |
Storage |
|
10x red blood cell lysate |
300 ml |
Store at RT for 18 months.
|
|
Nuclear lysate |
250 ml x4 |
|
|
Protein precipitation solution |
330 ml |
|
|
DNA lysate |
90 ml |
Precautions
1. All centrifugation steps are performed at room temperature using a conventional benchtop centrifuge that can spin up to 2,500 x g and is equipped with a 50ml heart tube rotor.
2. Prepare Isopropanol and 70% Ethanol.
