Blood/Tissue/Cell DNA extraction kit
Size: 200 T
Lot # Check on the product label
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Price: $430.00
Catalog# BPC1148
Size: 200 T
Lot # Check on the product label
Description
The Easyspin RNA Isolation Kit offers a fast and easy way to extract high quality RNA from tissues and cells. The whole process is phenol/chloroform-free. The unique lysis buffer immediately lyses biological samples and inactivates RNase and DNase. Ethanol is added to the lysate to provide appropriate binding conditions for RNA, and RNA selectively binds to the silica-membrane of the RNA column in the high-salt buffer. RNA is purified through a series of wash-spin steps to remove protein followed by elution of RNA from silica membrane with RNase-free H2O.
Kit Contents and Storage
|
Components |
Size |
Storage |
|
Buffer TL |
40 ml |
Store at RT for 12 months.
|
|
Buffer BB |
40 ml |
|
|
Buffer CB |
60 ml |
|
|
Buffer IR |
100 ml |
|
|
Buffer WB |
50 ml Add indicated ethanol before first use |
|
|
Elution Buffer |
15 ml x2 |
|
|
Proteinase K (20mg / ml) |
80 mg |
|
|
Adsorption column AC |
200 pcs |
|
|
Collection tube (2ml) |
200 pcs |
Note: Proteinase K is a lyophilizate. Centrifuge a few seconds and reconstitute with 1 ml distilled water, aliquot solution. Store at –20°C. Avoid repeated freezing and thawing.
Precautions
1. All centrifugation steps are completed at room temperature(can also be centrifuged at 4°C), using a traditional benchtop centrifuge with a speed of 13,000 rpm.
2. Prepare 1X PBS (phosphate buffered saline), and isopropanol.
