Polyclonal Anti- Caspase-3, CASP3 antibody

Catalog# YPA2068

Lot # Check on the product label

Size 100 μl

Isotype IgG

Host Rabbit

Reactivity

Human, mouse, rat, bovine, dog

Immunogen KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Caspase 3.

Purification Purified by immunogen affinity chromatography

Content Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Recommend Application

Western Blot, WB (1:500-1:1000)

Immunohistochemistry, IHC (1:100-1:200)

Immunocytochemistry, ICC/IF(1:100-500)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.

Avoid repeated freeze and thaw cycles.

Background

Caspase 3 (also known as CPP32/Yama/apopain) is a member of the cysteine-aspartic acid protease (caspase) family. It is formed from a 32 kDa zymogen that is cleaved into 17 kDa and 12 kDa subunits. Caspase-3 plays essential roles in programmed cell death, It has also been found to be necessary for normal brain development as well as its typical role in apoptosis, where it is responsible for chromatin condensation and DNA fragmentation. Elevated levels of a fragment of Caspase-3, p17, in the bloodstream is a sign of a recent myocardial infarction. It is now being shown that caspase-3 may play a role in embryonic and hematopoietic stem cell differentiation.

Reference

1. Abdul-Ghani M, Megeney LA (June 2008). "Rehabilitation of a contract killer: caspase-3 directs stem cell differentiation". Cell Stem Cell 2 (6): 515–6.

2. Alnemri ES, Livingston DJ, Nicholson DW, Salvesen G, Thornberry NA, Wong WW, Yuan J (October 1996). "Human ICE/CED-3 protease nomenclature". Cell 87 (2): 171.

3. Porter AG, Jnicke RU (February 1999). "Emerging roles of caspase-3 in apoptosis". Cell Death Differ. 6 (2): 99–104.