Polyclonal Anti- Matrix metalloproteinase 1, MMP-1 antibody

Catalog# YPA1459

Lot # Check on the product label

Size 100 μl

Isotype IgG

Host Rabbit

Reactivity 

Human

Immunogen Recombinant human MMP1 protein expressed in E.Coli.

Purification Immunogen affinity purified

Content In 0.01M PBS (pH7.4), 0.5% BSA, 30% Glycerol and 0.05% Sodium Azide.

Recommend Application

Western Blot, WB (1:250-1000)

ELISA (1:50-500)

Immunohistochemistry, IHC-P (1:20-200)

Immunocytochemistry, ICC/IF (1:20-200)

Flow Cytometry, Flow-Cyt (1:20-100)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.

Avoid repeated freeze and thaw cycles.

Background

Interstitial collagenase, also known as fibroblast collagenase and matrix metalloproteinase-1 (MMP-1), is an enzyme that in humans is encoded by the MMP1 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. MMP-1 was the first vertebrate collagenase both purified to homogeneity as a protein, and cloned as a cDNA. MMP-1 has an estimated molecular mass of 54 kDa.

Reference

1. Brinckerhoff CE, Ruby PL, Austin SD, Fini ME, White HD (February 1987). "Molecular cloning of human synovial cell collagenase and selection of a single gene from genomic DNA". J. Clin. Invest. 79 (2): 542–6.

2. Pendás AM, Santamaría I, Alvarez MV, Pritchard M, López-Otín C (October 1996). "Fine physical mapping of the human matrix metalloproteinase genes clustered on chromosome 11q22.3". Genomics. 37 (2): 266–8.

3. Gross J, Lapiere CM (June 1962). "Collagenolytic activity in amphibian tissues: a tissue culture assay". Proc. Natl. Acad. Sci. U.S.A. 48 (6): 1014–22.

4. Goldberg GI, Wilhelm SM, Kronberger A, Bauer EA, Grant GA, Eisen AZ (May 1986). "Human fibroblast collagenase. Complete primary structure and homology to an oncogene transformation-induced rat protein". J. Biol. Chem. 261 (14): 6600–5.