Polyclonal Anti- COL5A2 antibody

Catalog# YPA2531

Lot # Check on the product label

Size 100 μl

Isotype IgG

Host Rabbit

Reactivity 

Human, rat

Immunogen KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Collagen 5 alpha 2.

Purification Purified by immunogen affinity chromatography.

Content Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Recommend Application

Western Blot, WB (1:500-1:1000)

Immunohistochemistry, IHC (1:50-1:100)

Immunocytochemistry, ICC/IF(1:50-1:200)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.

Avoid repeated freeze and thaw cycles.

Background

COL5A2 encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. It shows predictive potential in myocardial infarction (MI), and in principle may represent a novel candidate marker for the identification and treatment of ischemic cardiovascular disease.

Reference

1. Azuaje F, Zhang L, Jeanty C, Puhl SL, Rodius S, Wagner DR. Analysis of a gene co-expression network establishes robust association between Col5a2 and ischemic heart disease. BMC Med Genomics. 2013 Apr 10;6:13.