Rat Total antioxidant capacity, T-AOC ELISA Kit

Catalog No.: BZEK1352

Size: 96T

Range: 1.5 U/ml - 24 U/ml

Sensitivity: 0.1 U/ml

Storage and Expiration: Store at 2-8℃ for 6 months. 

Application: For quantitative detection of T-AOC in rat serum, plasma, tissue homogenate or cell culture supernatant.

Introduction

Total antioxidant capacity is the term used to describe the ability of antioxidants in different foods to clean harmful free radicals in the blood and cells. Often referred to by the acronym TAC, it takes into account the amount of water-based and fat-based antioxidants present in food. Understanding this figure helps individuals decide which foods offer the greatest antioxidant benefit.

Principle of the Assay  

This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti- T-AOC antibody was pre-coated onto 96-well plates. And the HRP conjugated anti- T-AOC antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the T-AOC amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of T-AOC can be calculated.

Kit components

1. One 96-well plate pre-coated with anti- rat T-AOC antibody

2. Standard: 0.3 ml × 6 tubes (0 U/ml, 1.5 U/ml, 3 U/ml, 6 U/ml, 12 U/ml, 24 U/ml)

3. Wash buffer (20×): 25 ml. Dilution: 1:20

4. Sample diluent buffer: 6 ml

5. HRP conjugated anti- rat T-AOC antibody (RTU): 10 ml

6. Stop solution: 6 ml

7. TMB substrate A:  6ml

8. TMB substrate B:  6ml

9. Plate sealer:   2

10. Hermetic bag:  1

Material Required But Not Provided

1. 37℃ incubator
2. Microplate reader (wavelength: 450nm)
3. Precise pipette and disposable pipette tips
4. Automated plate washer
5. ELISA shaker
6. 1.5ml of Eppendorf tubes
7. Absorbent filter papers
8. Plastic or glass container with volume of above 1L