Polyclonal Anti- Nrf2 antibody

Catalog# YPA1865

Lot # Check on the product label

Size 100 μl

Isotype IgG

Host Rabbit

Reactivity 

Human, mouse, rat

Immunogen KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NRF2.

Purification Purified by immunogen affinity chromatography

Content Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Recommend Application

Western Blot, WB (1:500-1:1000)

Immunohistochemistry, IHC (1:50-1:200)

Immunocytochemistry, ICC/IF (1:100-500)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.

Avoid repeated freeze and thaw cycles.

Background

Nuclear factor (erythroid-derived 2)-like 2, also known as NFE2L2 or Nrf2, is a transcription factor that in humans is encoded by the NFE2L2 gene. Nuclear factor-erythroid 2-related factor 2 (Nrf-2) is associated with the regulation of tumor protein p53 (p53) and the apoptotic response of oral cancer cells. AGEs inhibit the expression of Nrf-2, p53 and Bcl-2 associated x apoptosis regulator, and increase the expression of apoptosis regulator Bcl-x protein. AGEs regulate the downstream pathways Nrf-2 and Bcl-xl via ERK phosphorylation. AGEs regulate the survival of oral cancer cells via Nrf-2 and Bcl-xl through p53 regulation, which explains the poor prognosis of patients with DM who have oral cancer.

Reference

1. Ko SY, Ko HA, Shieh TM, Chi TC, Chen HI, Chen YT, Yu YH, Yang SH, Chang SS. Advanced glycation end products influence oral cancer cell survival via Bcl-xl and Nrf-2 regulation <i>in vitro</i>. Oncol Lett. 2017 May;13(5):3328-3334.