Polyclonal Anti- Tryptophan 2,3 dioxygenase, TDO2 antibody

Catalog# YPA2534

Lot # Check on the product label

Size 100 μl

Isotype IgG

Host Rabbit

Reactivity 

Human, mouse, rat

Immunogen TDO2 fusion protein

Purification Antigen affinity purification

Content PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Recommend Application

Western Blot, WB (1:250-1:500)

Immunohistochemistry, IHC (1:50-1:200)

Immunofluorescence, IF (1:100-1:400)

Other applications have not been tested.

The optimal dilutions should be determined  by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.

Avoid repeated freeze and thaw cycles.

Background

In enzymology, tryptophan 2,3-dioxygenase (EC 1.13.11.11) is a heme enzyme that catalyzes the oxidation of l-tryptophan (l-Trp) to N-formyl-l-kynurenine, as the first and rate-limiting step of the kynurenine pathway. Tryptophan 2,3-dioxygenase plays a central role in the physiological regulation of tryptophan flux in the human body, as part of the overall biological process of tryptophan metabolism. TDO catalyses the first and rate-limiting step of tryptophan degradation along the kynurenine pathway and thereby regulates systemic tryptophan levels. In humans, tryptophan 2,3-dioxygenase is encoded by the TDO2 gene.

Reference

1. Pilotte L, Larrieu P, Stroobant V, Colau D, Dolusic E, Frédérick R, De Plaen E, Uyttenhove C, Wouters J, Masereel B, Van den Eynde BJ (February 2012). "Reversal of tumoral immune resistance by inhibition of tryptophan 2,3-dioxygenase". Proceedings of the National Academy of Sciences of the United States of America. 109 (7): 2497–2502. Bibcode:2012PNAS..109.2497P.

2. Zhang Y, Kang SA, Mukherjee T, Bale S, Crane BR, Begley TP, Ealick SE (January 2007). "Crystal structure and mechanism of tryptophan 2,3-dioxygenase, a heme enzyme involved in tryptophan catabolism and in quinolinate biosynthesis". Biochemistry. 46 (1): 145–155.