Monoclonal Anti- NFkB/ NFKB1 p105/ p50 antibody

Catalog# YMA1374

Lot # Check on the product label

Size 100 μl

Isotype IgG

Host Rabbit

Reactivity 

Human, mouse, rat

Immunogen A synthesized peptide derived from human NFkB/NFKB1 (p105/p50).

Purification Affinity-chromatography

Content In 0.01M PBS (pH7.4), 0.5% BSA, 30% Glycerol and 0.05% Sodium Azide.

Recommend Application

Western Blot, WB (1:250-1000)

Immunohistochemistry, IHC (1:20-100)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.

Avoid repeated freeze and thaw cycles.

Background

Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) is a family of transcription factor protein complexes that controls transcription of DNA, cytokine production and cell survival. NF-κB is found in almost all animal cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, heavy metals, ultraviolet irradiation, oxidized LDL, and bacterial or viral antigens. NF-κB plays a key role in regulating the immune response to infection. Incorrect regulation of NF-κB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. NF-κB has also been implicated in processes of synaptic plasticity and memory.

Reference

1. Gilmore TD (October 2006). "Introduction to NF-kappaB: players, pathways, perspectives". Oncogene. 25 (51): 6680–6684.

Brasier AR (2006). "The NF-kappaB regulatory network". Cardiovascular Toxicology. 6 (2): 111–130.

2. Perkins ND (January 2007). "Integrating cell-signalling pathways with NF-kappaB and IKK function". Nature Reviews. Molecular Cell Biology. 8 (1): 49–62.

3. Concetti J, Wilson CL (September 2018). "NFKB1 and Cancer: Friend or Foe". Cells. 7 (9): 133.

4. Gilmore TD (November 1999). "The Rel/NF-kappaB signal transduction pathway: introduction". Oncogene. 18 (49): 6842–6844.

Tian B, Brasier AR (2003). "Identification of a nuclear factor kappa B-dependent gene network". Recent Progress in Hormone Research. 58: 95–130.