Stool DNA Mini kit
Size: 50 T
Lot # Check on the product label
Application: For extraction of various stool DNA.
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Price: ---
Catalog# BPC1173
Size: 50 T
Lot # Check on the product label
Application: For extraction of various stool DNA.
Description
The DNA Stool Mini Kit provides fast and easy purification of total DNA from fresh or frozen stool samples. Purified DNA is of high quality and well suited for use in PCR and other downstream enzymatic reactions. The simple spin procedure yields pure DNA ready for direct use in less than 1 hour. Purification requires no phenol–chloroform extraction or alcohol precipitation, and involves minimal handling. DNA is eluted in low-salt buffer and is free of protein, nucleases, and other impurities or inhibitors. The purified DNA is ready for use in PCR and other enzymatic reactions, or can be stored at –20℃ for later use.
Stool samples typically contain many compounds that can degrade DNA and inhibit downstream enzymatic reactions. To ensure removal of these substances, the DNA Stool Mini Kit contains Buffer AB which efficiently adsorb these substances early in the purification process so that they can easily be removed by a quick centrifugation step. In addition, the kit contains Buffer ASL, which is specially developed to remove inhibitory substances from stool samples.
Kit Contents and Storage
|
Components |
Size |
Storage |
|
Equilibration Buffer |
5 ml |
Store at RT for 12 months. |
|
Buffer ASL |
70 ml |
|
|
Impurity remover AB |
5 ml |
|
|
Binding Buffer CB |
11 ml |
|
|
Inhibitor Remover IR |
25 ml |
|
|
Wash Buffer WB |
13 ml Add indicated ethanol before first use |
|
|
Elution buffer EB |
15 ml |
|
|
Proteinase K solution (20 mg/ml) |
1 ml |
Store at 4℃ for 12 months. |
|
Adsorbent columns with Collection Tubes |
50 pcs |
Store at RT for 12 months |
