54TRUEscript RT Kit (+gDNA Eraser)

Catalog# BPC1047-1
Lot # Check on the product label
Size: 50 Tx 20 μl
Storage and Expiration: Store at -20°C for 1 year.
Application: First strand cDNA synthesis. it can be used for the detection of high-copy and low-copy genes.
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Price: $200.00

  


Catalog# BPC1047-1


Lot # Check on the product label


Size: 50 Tx 20 μl


Storage and Expiration: Store at -20°C for 1 year.


Application: First strand cDNA synthesis. it can be used for the detection of high-copy and low-copy genes.


Description:

5×TRUE RT MasterMix II contains all the reagents (TRUEscript HˉRTase, RNase Inhibitor, Random primers, Oligo dT Primer, dNTP Mixture, Buffer) required for the first-strand reverse transcription. Usually Real Time RT-PCR and other experiments need to use DNase I to digest and remove the remaining genomic DNA (gDNA) in the RNA, but the traditional DNase I process is complicated and easily causes RNA degradation and loss.


Kit Components

 Component

Size

4×gDNA Eraser Mix

200 μl

TRUE RT MasterMix II

200 μl

No RTase Control Mix *

20 μl 

RNase free H2O

1 ml

* The components of 5×No RTase Control Mix and 5 × TRUE RT MasterMix II are exactly the same, but they do not contain TRUEscript Hˉ RTase reverse transcriptase, which can be used as a negative control for reverse transcription

Details