54TRUEscript RT Kit (+gDNA Eraser)
Lot # Check on the product label
Size: 50 Tx 20 μl
Storage and Expiration: Store at -20°C for 1 year.
Application: First strand cDNA synthesis. it can be used for the detection of high-copy and low-copy genes.
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Price: $200.00
Catalog# BPC1047-1
Lot # Check on the product label
Size: 50 Tx 20 μl
Storage and Expiration: Store at -20°C for 1 year.
Application: First strand cDNA synthesis. it can be used for the detection of high-copy and low-copy genes.
Description:
5×TRUE RT MasterMix II contains all the reagents (TRUEscript HˉRTase, RNase Inhibitor, Random primers, Oligo dT Primer, dNTP Mixture, Buffer) required for the first-strand reverse transcription. Usually Real Time RT-PCR and other experiments need to use DNase I to digest and remove the remaining genomic DNA (gDNA) in the RNA, but the traditional DNase I process is complicated and easily causes RNA degradation and loss.
Kit Components
Component |
Size |
4×gDNA Eraser Mix |
200 μl |
5×TRUE RT MasterMix II |
200 μl |
5×No RTase Control Mix * |
20 μl |
RNase free H2O |
1 ml |
* The components of 5×No RTase Control Mix and 5 × TRUE RT MasterMix II are exactly the same, but they do not contain TRUEscript Hˉ RTase reverse transcriptase, which can be used as a negative control for reverse transcription
Details
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