miRNA Real-Time PCR Assay kit

Catalog# BPC1054

Lot # Check on the product label

Size: 50 Tx 50 μl

Storage and Expiration: Store at -20°C in dark for 6 months. 2x miRNA qPCR Mix (With Sybr Green) can be stored at 4°C for short-term use to avoid repeated freeze and thaw cycles; Reverse primer (10μM) should be stored at -20°C after each use.

Note: This kit must be used in conjunction with miRNA first-strand cDNA synthesis kit (BPC1053).

Description:

The kit adopts the principle of SYBR® Green I chimeric fluorescence to carry out miRNA fluorescence quantitative detection. The kit contains all reagents for quantitative miRNA fluorescence detection, including 2×miRNA qPCR Mix, Reverse Primer. Among them, 2×miRNAqPCR Mix (With Sybr Green) is a new generation of premixed fluorescent quantitative PCR detection reagent specially developed for miRNA quantitative detection. The DNA Polymerase uses chemically modified hot-start polymerase, cooperating with a special buffer system, it has better reaction specificity, higher sensitivity, and accurate quantification can be carried out in a wider range.

Kit Components

Component Required But Not Provided

1. Molecular biology grade water (nuclease-free)

2. The qPCR upstream primer corresponding to the miRNA to be detected (Forward primer)