PCR Enhancer

Catalog# BPC1034-1

Lot # Check on the product label

Size: 500 μl

Storage and Expiration: Store at RT.

Description: PCR Enhancer can cooperate with thermostable DNA polymerases to promote the effective amplification of various DNA templates and increase the sensitivity and specificity of PCR reactions. In PCR reactions, PCR Enhancer mainly works by improving the thermal stability of DNA polymerase and reducing the secondary structure of DNA. Therefore, for complex templates (such as high GC content), PCR Enhancer can greatly reduce the influence of DNA secondary structure on PCR by increasing annealing temperature, while the activity of enzyme is not changed.

Protocol: The final concentration of PCR Enhancer in the PCR reaction system is 2%-8%, and the usual concentration is 4%-6%. Users can adjust the final concentration.

Example: In the final volume of the 50µl PCR reaction system, add 2µl PCR Enhancer, the final concentration of PCR Enhancer is 4%.