T4 DNA Ligase

Catalog# BPC1101-1

Lot # Check on the product label

Size: 100 U

Storage: Store at -20°C for 6 months. 

Concentration: 5 U/µl

Application:

(1) Quick connection between DNA fragments and carrier DNA;

(2) Cloning of restriction enzyme cut fragments;

(3) Connect the DNA fragment with Linker or Adaptor.

Description: T4 DNA Ligase is isolated and purified from Escherichia coli expressing T4 DNA Ligase gene after induction. It catalyzes the phosphodiester binding of the 5'-phosphate and 3' hydroxyl ends of adjacent DNA strands. The enzyme catalyzes the ligation of blunt-ended or sticky-ended DNA and repairs single-stranded nicks in single-stranded DNA in double-stranded DNA, RNA, and DNA/RNA hybrids.

Enzyme storage buffer: 20mM Tris-HCl (pH7.5), 0.1 mM EDTA, 1mM DTT, 50 mM KCl, 50% glycerol.

10× Ligation Buffer: 400 mM Tris-HCl, 100 mM MgCl 2, 100 mM DTT, 5 mM ATP(PH7.8), other components.

• Recommended ligation reaction system(Take a 10 μl reaction system as an example):

1. PCR reaction cycle settings:

Sticky end ligation: 22°C reaction for 10 minutes (can be extended to 1 hour as needed);

Blunt-end ligation: 22°C for 2 hours or 16°C overnight.