Monoclonal Anti-E2b Tag Antibody

Catalog# BTL1004

Lot # Check on the product label

Size 100μg

Isotype IgG1

Host Mouse

Reactivity All

Specificity Recognize C-terminal, internal and N-terminal E2b tagged proteins.

Product Form Liquid (1mg/ml)

Immunogen 

A synthetic peptide GVSSTSSDFRDR conjugated to KLH. 

Recommend Application

Western Blot (1:1000-10000)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.

Storage Instruction 

Store at -20°C for 1 year. Or aliquot and store at -80°C for long term.

Avoid repeated freeze and thaw cycles.

Background

E2 Tags are 10-amino acids (epitope E2a: SSTSSDFRDR)- and 12 amino acids (epitope E2b: GVSSTSSDFRDR)-long peptides derived from the E2 protein of bovine papillomavirus type 1. The modules for C-terminal tagging with E2a and E2b epitopes were constructed by the modification of the pYM-series plasmid. The N-terminal E2a and E2b tagging modules were based on pOM-series plasmid.

Reference

1. Tiina Tamm. Plasmids with E2 epitope tags: tagging modules for N- and C-terminal PCR-based gene targeting in both budding and fission yeast, and inducible expression vectors for fission yeast. Yeast Volume 26, Issue 1, pages 55–66, January 2009.