Monoclonal Anti-Flag / DYKDDDDK Tag Antibody

Catalog# BTL1005

Lot # Check on the product label

Size 100μg

Isotype IgG1

Host Mouse

Reactivity All

Product Form Liquid (1mg/ml)

Immunogen 

A synthetic peptide DYKDDDDK conjugated to KLH.

Recommend Application

Western Blot (1:1000-10000)

Immunofluorescence, IF (1:200-1000)

Immunoprecipitation, IP (1:100-500)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.

Storage Instruction 

Store at 2-8°C for 1 month. Or aliquot and store at -20°C or below for long term. Avoid repeated freeze and thaw cycles.

Background

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. The peptide sequence of the FLAG-tag is: N-DYKDDDDK-C (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein.