Monoclonal Anti-mCherry Tag Antibody

Catalog# BTL1015

Lot # Check on the product label

Size 100μg

Isotype IgG1

Host Mouse

Reactivity All

Specificity

Detect the expression level of mCherry tag fusion proteins.                             

Product Form Liquid (1mg/ml)

Immunogen 

Recombinant mCherry protein with full length.

Recommend Application

Western Blot (1:1000-10000)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.

Storage Instruction 

Store at -20°C for 1 year. Or aliquot and store at -80°C for long term.

Avoid repeated freeze and thaw cycles.

Background

mCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. It matures quickly, with a t_0.5 of 15 minutes, allowing it to be visualised soon after translation.

Reference

1. Shaner, Nathan C; Campbell, Robert E; Steinbach, Paul A; Giepmans, Ben N G; Palmer, Amy E; Tsien, Roger Y (2004). "Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. Red fluorescent protein". Nature Biotechnology 22 (12): 1567–72.