Monoclonal Anti-SUMO Tag Antibody

Catalog# BTL1019

Lot # Check on the product label

Size 100μg

Isotype IgG1

Host Mouse

Reactivity All

Specificity

Detect the expression level of SUMO tag fusion proteins.

Product Form Liquid (1mg/ml)

Immunogen 

Purified recombinant yeast SUMO.

Recommend Application

Western Blot (1:1000-10000)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.                                                                                                                        

Storage Instruction 

Store at -20°C for 1 year. Or aliquot and store at -80°C for long term.

Avoid repeated freeze and thaw cycles.

Background

Small Ubiquitin-like Modifier (or SUMO) proteins are a family of small proteins that are covalently attached to and detached from other proteins in cells to modify their function. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle. SUMO proteins are similar to ubiquitin, and SUMOylation is directed by an enzymatic cascade analogous to that involved in ubiquitination. In contrast to ubiquitin, SUMO is not used to tag proteins for degradation.

Reference

1. Hay RT (Apr 2005). "SUMO: a history of modification". Mol. Cell 18 (1): 1–12.