Monoclonal Anti-T7 Tag Antibody

Catalog# BTL1020

Lot # Check on the product label

Size 100 μg

Isotype IgG1

Host Mouse

Reactivity All

Product Form Liquid (1 mg/ml)

Immunogen 

A synthetic peptide MASMTGGQQMG conjugated to KLH.

Recommend Application

Western Blot (1:1000-10000)

Immunofluorescence, IF (1:100-1:1000)

Immunoprecipitation, IP (1:100-1:1000)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.                                                                                                                        

Storage Instruction 

Store at -20°C for 1 year. Or aliquot and store at -80°C or long term.

Avoid repeated freeze and thaw cycles.

Background

The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene 10. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems.

Reference

Studier FW, Moffatt BA. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol. 1986 May 5;189(1):113-30