Monoclonal Anti-Tag-100 Tag Antibody

Catalog# BTL1021

Lot # Check on the product label

Size 100 μg

Isotype IgG1

Host Mouse

Reactivity All

Specificity

Recognize C-terminal, internal and N-terminal Tag-100 tagged proteins.

Product Form Liquid (1mg/ml)

Immunogen 

A synthetic peptide EETARFQPGYRS conjugated to KLH.

Recommend Application

Western Blot (1:1000-10000)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.                                                                                                                        

Storage Instruction 

Store at -20°C for 1 year. Or aliquot and store at -80°C for long term.

Avoid repeated freeze and thaw cycles.

Background

In molecular biology, extracellular-signal-regulated kinases (ERKs) or classical MAP kinases are widely expressed protein kinase intracellular signalling molecules that are involved in functions including the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. ERKs are known to activate many transcription factors, such as ELK1, and some downstream protein kinases. Disruption of the ERK pathway is common in cancers, especially Ras, c-Raf and receptors such as HER2. Tag-100 tag is an epitope tag composed of a 12-residue peptide, EETARFQPGYRS, derived from the C-termini of mammalian MAPK/ERK kinases.

Reference

1. Rao VN, Reddy ES (July 1994). "elk-1 proteins interact with MAP kinases". Oncogene 9 (7): 1855–60.