Monoclonal Anti-V5 Tag Antibody

Catalog# BTL1022

Lot # Check on the product label

Size 100μg

Isotype IgG1

Host Mouse

Reactivity All

Product Form Liquid (1mg/ml)

Immunogen 

A synthetic peptide GKPIPNPLLGLDST conjugated to KLH.

Recommend Application

Western Blot (1:1000-10000)

Immunoprecipitation, IP (1:100-500)

Immunofluorescence, IF (1:200-1000)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.                                                                                                                        

Storage Instruction 

Store at 2-8°C for 1 month. Or aliquot and store at -20°C or below for long term.

Avoid repeated freeze and thaw cycles.

Background

Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, c-myc-tag, and HA-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification. The V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).