Monoclonal Anti- Actin antibody

Catalog# BTL1025

Lot # Check on the product label

Size 100μg

Isotype IgG2b

Host Mouse

Reactivity

Human, mouse, rat, monkey, Chinese hamster, dog, spodoptera frugiperda

Specificity

Detect endogenous level of Actin protein.

Product Form Liquid

Immunogen 

Human Actin protein with full length. 

Recommend Application

Western Blot (1:5000)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Store at -20°C for 1 year.

Avoid repeated freeze and thaw cycles.

Background

Actin is a globular, roughly 42-kDa multi-functional protein found in all eukaryotic cells (the only known exception being nematode sperm), where it may be present at concentrations of over 100 μM. It is also one of the most highly-conserved cytoskeletal proteins that play fundamental roles in nearly all aspects of eukaryotic cell biology. Actin polymerization is essential for cell locomotion and is thought to generate the force responsible for cellular protrusions.

Reference

1. Leisel, T. P., Boujemaa, R., Pantaloni, D., Carlier, M.-F. Reconstitution of actin-based motility of Listeria and Shigella using pure proteins. Nature 401: 613-616, 1999.