Polyclonal Anti- β-Actin antibody

Catalog# BTL1026

Lot # Check on the product label

Size 100 μg

Isotype IgG

Host Rabbit

Reactivity

Human, mouse, rat, monkey, chicken, pig, cow, rabbit, D. Melanogaster

Specificity

Detect endogenous level of total β-Actin protein.

Product Form Liquid

Immunogen 

A synthetic peptide (conjugated to KLH) corresponding to N-terminal residues of human β-Actin.

Recommend Application

Western Blot (1:1500-2000)

Immunohistochemistry,IHC-P(1:100-1000)

ELISA (1:1000-5000)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Store at -20°C for 1 year.

Avoid repeated freeze and thaw cycles.

Background

Beta-actin (gene name ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Karakozova et al. (2006) found that approximately 40% of intracellular beta-actin is arginylated in vivo. In both wildtype and Ate1-null cells beta-actin was stable, suggesting that arginylation does not induce beta-actin degradation. And arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.¹ Nitric oxide (NO) is a paracrine mediator of vascular and platelet function that is produced in the vasculature by NO synthase-3. And beta-actin plays a critical role in regulating NO formation and signaling in platelets.² Beta actin is also usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western Blotting.

Reference

1. Karakozova, M., Kozak, M., Wong, C. C. L., Bailey, A. O., Yates, J. R, III, Mogilner, A., Zebroski, H., Kashina, A. Arginylation of beta-actin regulates actin cytoskeleton and cell motility. Science 313: 192-196, 2006.

2. Ji, Y., Ferracci, G., Warley, A., Ward, M., Leung, K.-Y., Samsuddin, S., Leveque, C., Queen, L., Reebye, V., Pal, P., Gkaliagkousi, E., Seager, M., Ferro, A. Beta-actin regulates platelet nitric oxide synthase 3 activity through interaction with heat shock protein 90. Proc. Nat. Acad. Sci. 104: 8839-8844, 2007.