Monoclonal Anti- GAPDH Antibody

Catalog# BTL1030

Lot # Check on the product label

Size 100 μg

Host Mouse

Reactivity

Human, mouse, rat, dog, rabbit, pig, chicken

Does not react with non-vertebrate and prokaryote species.

Product Form Liquid

Immunogen 

Recombinant human GAPDH protein.

Recommend Application

Western Blot (1:100-1000)

Immunoprecipitation, IP (1-2 μg per 100-500 μg of total protein. 1ml of cell lysate)

Immunofluorescence, IF (1:50-500)

Immunohistochemistry, IHC (1:50-500)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Store at -20°C for 1 year.

Avoid repeated freeze and thaw cycles.

Background

Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, and ER to Golgi vesicle shuttling.

Reference

1. Meyer-Siegler, K., Mauro, D. J., Seal, G., Wurzer, J., deRiel, J. K., Sirover, M. A. A human nuclear uracil DNA glycosylase is the 37-kDa subunit of glyceraldehyde-3-phosphate dehydrogenase. Proc. Nat. Acad. Sci. 88: 8460-8464, 1991.

2. Zheng L, Roeder RG, Luo Y (2003). "S phase activation of the histone H2B promoter by OCA-S, a coactivator complex that contains GAPDH as a key component". Cell 114 (2): 255–66.

3. A. Tarze, A. Deniaud, M. Le Bras, E. Maillier, D. Molle, N. Larochette, N. Zamzami, G. Jan, G. Kroemer, and C. Brenner (2007). "GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilization". Oncogene 26 (18): 2606–2620.