Polyclonal Anti- GAPDH antibody

Catalog# BTL1031

Lot # Check on the product label

Size 100 μl

Host Rabbit

Reactivity

Human, mouse, rat

Specificity

Detect the endogenous levels of total GAPDH protein.

Product Form Liquid

Immunogen 

Human GAPDH recombinant protein with full length.

Recommend Application

Western Blot (1:5000-10,000)

Immunohistochemistry, IHC (1:50-200)

Immunocytochemistry, ICC (1:50-200)

Flow Cytometry (1:50-100) Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Aliquot and store at -20°C for 1 year.

Avoid repeated freeze and thaw cycles.

Background

Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, and ER to Golgi vesicle shuttling.

Reference

1. Meyer-Siegler, K., Mauro, D. J., Seal, G., Wurzer, J., deRiel, J. K., Sirover, M. A. A human nuclear uracil DNA glycosylase is the 37-kDa subunit of glyceraldehyde-3-phosphate dehydrogenase. Proc. Nat. Acad. Sci. 88: 8460-8464, 1991.

2. Zheng L, Roeder RG, Luo Y (2003). "S phase activation of the histone H2B promoter by OCA-S, a coactivator complex that contains GAPDH as a key component". Cell 114 (2): 255–66.

3. A. Tarze, A. Deniaud, M. Le Bras, E. Maillier, D. Molle, N. Larochette, N. Zamzami, G. Jan, G. Kroemer, and C. Brenner (2007). "GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilization". Oncogene 26 (18): 2606–2620.