Polyclonal Anti- Lamin B Antibody

Catalog# BTL1034

Lot # Check on the product label

Size 100μg

Host Rabbit

Reactivity

Human, mouse, rat, rabbit, pig, cat, chicken

Product Form Liquid

Immunogen 

Purified Lamin B.

Recommend Application

Western Blot (1:100-400)

Immunohistochemistry, IHC-C (1:100-500)

Immunohistochemistry, IHC-P (1:50-200)

Immunohistochemistry, IHC-Fr(1:100-500)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Store at -20°C for 1 year.

Avoid repeated freeze and thaw cycles.

Background

Lamins are the major components of the nuclear lamina which underlies the nuclear envelope of eukaryotic cells , they are members of the intermediate filament protein family. Vertebrate lamins are classified into 2 types, A and B. Lamin B is expressed in all kinds of cells. It is a component of the interphase nuclear lamina, is required to maintain nuclear shape and mechanical integrity. Lamin B has a role in spindle assembly. Furthermore, lamin B was essential for the formation of the mitotic matrix that tethers a number of spindle assembly factors.

Reference

1. Tsai, M.-Y., Wang, S., Heidinger, J. M., Shumaker, D. K., Adam, S. A., Goldman, R. D., Zheng, Y. A mitotic lamin B matrix induced by RanGTP required for spindle assembly. Science 311: 1887-1893, 2006.

2. Goldman, R. D., Gruenbaum, Y., Moir, R. D., Shumaker, D. K., Spann, T. P. Nuclear lamins: building blocks of nuclear architecture. Genes Dev. 16: 533-547, 2002.

3. Maeno, H., Sugimoto, K., Nakajima, N. Genomic structure of the mouse gene (Lmnb1) encoding nuclear lamin B1. Genomics 30: 342-346, 1995.