Monoclonal Anti- α-Tubulin Antibody

Catalog# BTL1036

Lot # Check on the product label

Size 100μg

Isotype IgG1

Host Mouse

Reactivity

Human, mouse, rat, dog, rabbit, pig, cat, chicken, sea urchin, chlamydomonas

Does not react with bovine and yeast.

Specificity

Recognize the C-terminal of α-Tubulin.

Product Form Liquid

Immunogen 

Purified human α-Tubulin protein.

Recommend Application

Western Blot (1:100-1000)

Immunoprecipitation, IP (1:100)

Immunofluorescence, IF (1:500)

Immunohistochemistry, IHC (1:500)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Store at 4°C for 1 year.

Avoid repeated freeze and thaw cycles.

Background

Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 kiloDaltons. Microtubules are assembled from dimers of α- and β-tubulin. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8.

Reference

1. Williams RC Jr, Shah C, Sackett D (November 1999). "Separation of tubulin isoforms by isoelectric focusing in immobilized pH gradient gels". Anal Biochem 275 (2): 265–7.