Mouse Activin A ELISA Kit

Catalog No.: BEK1003

Size: 96T

Range: 15.6 pg/ml - 1000 pg/ml

Sensitivity < 7.8 pg/ml

Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months. 

Application: For quantitative detection of Activin A in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.

Introduction

Activin A, the homodimer of the beta-A subunit, is a cytokine member of the transforming growth factor-beta superfamily. It is produced in the gonads, pituitary gland, placenta, and other organs, and is expressed locally by the mesenchymal component of the hemopoietic microenvironment. It had a mitogenic effect on mouse osteoblastic cells and suppressed their alkaline phosphatase activity. It also has an important role in the inflammatory response and that FST may have significant therapeutic potential to reduce the severity of inflammatory diseases.

Principle of the Assay  

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Activin A polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Activin A polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Activin A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Activin A can be calculated.

Kit components

1. One 96-well plate pre-coated with anti-mouse Activin A antibody
2. Lyophilized mouse Activin A standards: 2 tubes (10ng / tube)                     
3. Sample / Standard diluent buffer: 30ml
4. Biotin conjugated anti-mouse Activin A antibody (Concentrated): 130μl. Dilution: 1:100
5. Antibody diluent buffer: 12ml
6. Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
7. ABC diluent buffer: 12ml
8. TMB substrate: 10ml
9. Stop solution: 10ml
10. Wash buffer (25X): 30ml
11. Note: Reconstitute standards and test samples with Kit Component 3.

Material Required But Not Provided

1. 37℃ incubator
2. Microplate reader (wavelength: 450nm)
3. Precise pipette and disposable pipette tips
4. Automated plate washer
5. ELISA shaker
6. 1.5ml of Eppendorf tubes
7. Plate cover
8. Absorbent filter papers
9. Plastic or glass container with volume of above 1L