Polyclonal Anti- Ubiquitin antibody

Catalog# YPA2811

Lot # Check on the product label

Size 100 μl

Isotype IgG

Host Rabbit

Reactivity 

Human, mouse, rat

Immunogen A synthesized peptide derived from human UBA52, corresponding to a region within the internal amino acids.

Purification  Purified by peptide affinity chromatography

Content Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Recommend Application

Western Blot, WB (1:250-1000)

Immunohistochemistry, IHC (1:20-100)

Immunocytochemistry, ICC/IF (1:50-250)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at 20°C for one year.

Avoid repeated freeze and thaw cycles.

Background

Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains).                       

Reference

1. Zhang P et al. Endometrial cancer-associated mutants of SPOP are defective in regulating estrogen receptor-a protein turnover. Cell Death Dis 6:e1687 (2015).

2. Ye X et al. Characterization of PHB1 and its role in mitochondrial maturation and yolk platelet degradation during development of Artemia embryos. PLoS One 9:e109152 (2014).