In Vivo Recombinant Monoclonal Anti- Mouse PD1 antibody

Catalog# BVA1001

Lot # Check on the product label

Size 1 mg

Clone # RMP1-14

Isotype IgG

Reactivities Mouse

Isotype Rat IgG2a, kappa

Immunogen The original rat hybridoma (Clone# RMP1-14) was generated by immunizing Sprague Dawley rats with mouse PD-1-transfected BHK

cells and using a P3U1 myeloma as the fusion partner.

Formulation
0.2 μM filtered solution of 1x PBS

Purity >95% by SDS-PAGE

Endotoxin level <1 EU/μg by LAL test

Recommend Application

Western blot, WB

Immunohistochemistry, IHC

Flow Cytometry, Flow-Cyt

In vitro and in vivo functional assays

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C to -70°C for one year.

Avoid repeated freeze and thaw cycles.

Background

The rat anti-mouse PD-1 monoclonal antibody reacts with the mouse PD-1 protein (CD279 or programmed death-1) encoded by the mouse pdcd1 gene, a member of the CD28 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2, both of which belong to the B7 family. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD 1 antibody and the anti-mouse PD L1 antibody which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The RMP1-14 antibody blocks the binding of both the mouse PD-L1 protein and the mouse PD-L2 protein to the mouse PD-1 protein.