In Vivo Recombinant Monoclonal Anti-Mouse PD-L1 antibody

Catalog# BVA1002

Lot # Check on the product label

Size 1 mg

Clone# 10F.9G2

Isotype IgG

Reactivities Mouse

Isotype Rat IgG2a, kappa

Immunogen The original rat hybridoma (Clone# 10F.9G2) was generated by immunizing rats with the mouse PD-L1 cDNA and mouse PD-L1 CHO transfectants.

Formulation
0.2 μM filtered solution of 1x PBS

Purity >95% by SDS-PAGE

Endotoxin level <1 EU/μg by LAL test

Recommend Application

Immunohistochemistry, IHC

Flow Cytometry, Flow-Cyt

In vitro and in vivo functional assays

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C to -70°C for one year.

Avoid repeated freeze and thaw cycles.

Background

The rat anti-mouse PD-L1 monoclonal antibody 10F.9G2 (rat IgG2b kappa) reacts with the mouse PD-L1 protein (programmed death ligand-1, B7-H1 or CD274), a member of the B7 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD-1 and anti-mouse PD-L1 antibodies which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The 10F.9G2 monoclonal antibody blocks the binding of the mouse PD-L1 protein to the mouse PD-1 protein.