Total Antioxidant Capacity, TAC Assay Kit (Colorimetric Method)
Size: 100 T / 50 samples
Range: 0.2-55.2 U/ml
Detection limit: 0.2 U/ml
Application: For detection the TAC in serum, plasma, red or cultured cells, culture medium, animal or plant tissue homogenate.
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Price: ---
Catalog No.: BYEK2200
Size: 100 T / 50 samples
Range: 0.2-55.2 U/ml
Detection limit: 0.2 U/ml
Application: For detection the TAC in serum, plasma, red or cultured cells, culture medium, animal or plant tissue homogenate.
Storage and Expiration: Store at 2-8℃ for 6 months.
Introduction
Total antioxidant capacity (TAC) determinations are simple, inexpensive, and able to evaluate the capacity of known and unknown antioxidants and their additive, synergistic and/or antagonistic actions, in chemical and biological systems. However, different TAC assays correlate poorly with each other; since each TAC assay is sensitive to a particular combination of compounds, but exclude many others. The TAC values for foods cannot be translated to the in vivo (human) antioxidant defenses, and furthermore, to health effects provided by that food. Alcoholism causes an impaired antioxidant capacity and a decreased secretion of amylase, which is ameliorated due to the alcohol withdrawal regimen . The strong correlation between blood and saliva with respect to the antioxidants suggests the potential future use of saliva as a laboratory tool in clinical medicine.
Kit Component
|
Reagent |
Format |
Size |
Storage |
|
Reagent A |
Liquid |
60 ml x 2 |
2-8℃ |
|
Reagent B |
Powder |
2 vials |
2-8℃ |
|
Before use, reconstitute each vial with 120 ml of double distilled water, if the powder is hard to dissolve, end users can do 37℃ water bath. |
|||
|
Reagent C |
Liquid |
5 ml x 1 |
2-8℃ in dark |
|
Reagent C diluent buffer |
Liquid |
60 ml x 1 |
2-8℃ |
|
Before use, dilute Reagent C with Reagent C diluent buffer at 1:19, i.e. Add 1ml of Reagent C into 18ml of Reagent C diluent buffer. (Reagent C working solution) |
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|
Reagent D |
Liquid |
24 ml x 1 |
Room temperature |
|
Reagent E |
Liquid |
24 ml x 1 |
Room temperature |
|
Reagent E may freeze for the low temperature, before use, 37℃ water bath until get the transparency liquid. |
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Material Required But Not Provided
1. 37℃ incubator
2. Microplate reader or spectrophotometer (wavelength: 520 nm)
3. Precise pipette and disposable pipette tips
4. Centrifuge
5. Vortex mixer
