Monkey Interleukin-6, IL-6 ELISA Kit

Catalog No.: BZEK2864

Size: 96T

Range: 25 ng/L - 400 ng/L

Sensitivity: 2 ng/L

Storage and Expiration: Store at 2-8℃ for 6 months. 

Application: For quantitative detection of IL-6 in monkey serum, plasma, tissue homogenate or cell culture supernatant.

Introduction

Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In humans, it is encoded by the IL6 gene. In addition, osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. IL-6's role as an anti-inflammatory myokine is mediated through its inhibitory effects on TNF and IL-1 and its activation of IL-1ra and IL-10. There is some early evidence that IL-6 can be used as an inflammatory marker for severe COVID-19 infection with

poor prognosis, in the context of the wider coronavirus pandemic.

Principle of the Assay  

This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti- IL-6 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti- IL-6 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-6 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-6 can be calculated.

Kit components

1. One 96-well plate pre-coated with anti- monkey IL-6 antibody

2. Standard: 0.5 ml (800 ng/L)

3. Standard diluent buffer: 1.5 ml

4. Wash buffer (30×): 20 ml. Dilution: 1:30

5. Sample diluent buffer: 6 ml

6. HRP conjugated anti- monkey IL-6 antibody (RTU): 6ml

7. Stop solution: 6 ml

8. TMB substrate A: 6 ml

9. TMB substrate B: 6 ml

10. Plate sealer: 2

11. Hermetic bag: 1

Material Required But Not Provided

1. 37℃ incubator
2. Microplate reader (wavelength: 450nm)
3. Precise pipette and disposable pipette tips
4. Automated plate washer
5. ELISA shaker
6. 1.5ml of Eppendorf tubes
7. Absorbent filter papers
8. Plastic or glass container with volume of above 1L