Human Interleukin-6, IL-6 HiLA Kit

Catalog No.: BHK1007

Size: 100 T

Detection Range: 0 pg/ml - 30,000 pg/ml

Linear Range: 0.13 pg/ml - 3000 pg/ml

Storage and Expiration: Store at 2-8℃ in dark for 12 months.

Application: For quantitative detection of human IL-6 in buffer solution, serum, cell culture supernatant.

Introduction

Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In humans, it is encoded by the IL6 gene. In addition, osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. IL-6's role as an anti-inflammatory myokine is mediated through its inhibitory effects on TNF and IL-1 and its activation of IL-1ra and IL-10.

Principle of the Assay  

Incubate the acceptor beads coated with anti- IL-6 antibody 1, biotin conjugated anti- IL-6 antibody 2, and the sample to form a sandwich immune complex (antibody 1-sample-antibody 2). The immune complex is then incubated with donor beads coated with streptavidin to form a chemiluminescent complex, ensuring that the distance between the two beads is less than 200 nm. When the distance between the two beads is less than 200 nm, the donor beads is excited by light (680 nm), producing singlet oxygen which diffuses to the acceptor beads, inducing energy transfer. The acceptor beads absorb this energy and emit scattering light at 615 nm. The light signal is collected by a photosensitive detector, and the concentration of IL-6 in the sample is calculated through mathematical fitting. In the absence of human IL-6 in the sample, no immune complex is formed, or the distance between the two beads exceeds 200 nm, exceeding the transfer distance for singlet oxygen, and the acceptor beads do not emit any light.

Kit components

Material Required But Not Provided

1. Deionized water
2. Luminescence plate or strip
3. Homogeneous chemiluminescence analyzer, or multimode microplate reader with Alpha module

Precautions

1. It is recommended to aliquot the reconstituted standard and store at -20℃, the series of gradient standards should be used within 2 h. Avoid repeated freeze-thaw cycles.
2. It is recommended that the dilution matrix for the standard be consistent with the dilution matrix of the sample to be tested.
3. Do not use the expired components and the components from different batches.
4. For your safety and health, please wear the lab coat and disposable gloves to operate.

Reference

1. Ferguson-Smith AC, Chen YF, Newman MS, May LT, Sehgal PB, Ruddle FH (April 1988). "Regional localization of the interferon-beta 2/B-cell stimulatory factor 2/hepatocyte stimulating factor gene to human chromosome 7p15-p21". Genomics. 2 (3): 203–208.