Human Interleukin-22, IL-22 HiLA Kit

Catalog No.: BHK1014

Size: 100 T

Detection Range: 0 pg/ml - 1,000,000 pg/ml

Linear Range: 15.8 pg/ml - 30,000 pg/ml

Storage and Expiration: Store at 2-8℃ in dark for 12 months.

Application: For quantitative detection of human IL-22 in buffer solution, serum, cell culture supernatant.

Introduction

Interleukin-22 (IL-22) is a protein that in humans is encoded by the IL22 gene. IL-22 is an α-helical cytokine. IL-22 binds to a heterodimeric cell surface receptor composed of IL-10R2 and IL-22R1 subunits. IL-22R is expressed on tissue cells, and it is absent on immune cells. Crystallization is possible if the N-linked glycosylation sites are removed in mutants of IL-22 bound with high-affinity cell-surface receptor sIL-22R1. The crystallographic asymmetric unit contained two IL-22-sIL-22R1 complexes.

Principle of the Assay  

Incubate the F conjugated anti- IL-22 antibody 1, biotin conjugated anti- IL-22 antibody 2, acceptor beads coated with F-conjugate, and the sample to form a sandwich immune complex (antibody 1-sample-antibody 2). The immune complex is then incubated with donor beads coated with streptavidin to form a chemiluminescent complex, ensuring that the distance between the two beads is less than 200 nm. When the distance between the two beads is less than 200 nm, the donor beads is excited by light (680 nm), producing singlet oxygen which diffuses to the acceptor beads, inducing energy transfer. The acceptor beads absorb this energy and emit scattering light at 615 nm. The light signal is collected by a photosensitive detector, and the concentration of IL-22 in the sample is calculated through mathematical fitting. In the absence of human IL-22 in the sample, no immune complex is formed, or the distance between the two beads exceeds 200 nm, exceeding the transfer distance for singlet oxygen, and the acceptor beads do not emit any light.

Kit components

Material Required But Not Provided

1. Deionized water
2. Luminescence plate or strip
3. Homogeneous chemiluminescence analyzer, or multimode microplate reader with Alpha module

Precautions

1. It is recommended to aliquot the reconstituted standard and store at -20℃, the series of gradient standards should be used within 2 h. Avoid repeated freeze-thaw cycles.
2. It is recommended that the dilution matrix for the standard be consistent with the dilution matrix of the sample to be tested.
3. Do not use the expired components and the components from different batches.
4. For your safety and health, please wear the lab coat and disposable gloves to operate.

Reference

1. Dumoutier L, Van Roost E, Colau D, Renauld JC (August 2000). "Human interleukin-10-related T cell-derived inducible factor: molecular cloning and functional characterization as an hepatocyte-stimulating factor". Proceedings of the National Academy of Sciences of the United States of America. 97 (18): 10144–9.
2. Xie MH, Aggarwal S, Ho WH, Foster J, Zhang Z, Stinson J, et al. (October 2000). "Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL-22R". The Journal of Biological Chemistry. 275 (40): 31335–9.
3. PDB: 3DGC​; Jones BC, Logsdon NJ, Walter MR (September 2008). "Structure of IL-22 bound to its high-affinity IL-22R1 chain". Structure. 16 (9): 1333–44.
4. Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R (August 2004). "IL-22 increases the innate immunity of tissues". Immunity. 21 (2): 241–54.