Recombinant Rabbit Monoclonal Anti- SOX9 antibody

Catalog# BRM1117-1

Lot # Check on the product label

Size 50 μl

Isotype IgG

Host Rabbit

Reactivity 

Human, mouse, rat

Immunogen Recombinant protein of human SOX9.

Clone ID  4K8

Synonyms CMD1; CMPD1; SOX 9; SRA 1; SRA1; SRXX2; SRXY10; SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal;

Content Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.

Recommend Application

Western Blot, WB (1:1000-5000)

Immunohistochemistry,IHC-P (1:200-1000)
Immunocytochemistry,ICC/IF(1:200-1000)

Flow Cytometry, Flow-Cyt (1:100)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.

Avoid repeated freeze and thaw cycles.

Background

Transcription factor SOX-9 is a protein that in humans is encoded by the SOX9 gene. SOX-9 recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It is expressed by proliferating but not hypertrophic chondrocytes that is essential for differentiation of precursor cells into chondrocytes and, with steroidogenic factor 1, regulates transcription of the anti-Müllerian hormone (AMH) gene.

Reference

1. Tommerup N, Schempp W, Meinecke P, Pedersen S, Bolund L, Brandt C, et al. (June 1993). "Assignment of an autosomal sex reversal locus (SRA1) and campomelic dysplasia (CMPD1) to 17q24.3-q25.1". Nature Genetics. 4 (2): 170–4.

2. De Santa Barbara P, Bonneaud N, Boizet B, Desclozeaux M, Moniot B, Sudbeck P, et al. (November 1998). "Direct interaction of SRY-related protein SOX9 and steroidogenic factor 1 regulates transcription of the human anti-Müllerian hormone gene". Molecular and Cellular Biology. 18 (11): 6653–65.

3. Moniot B, Declosmenil F, Barrionuevo F, Scherer G, Aritake K, Malki S, et al. (June 2009). "The PGD2 pathway, independently of FGF9, amplifies SOX9 activity in Sertoli cells during male sexual differentiation". Development. 136 (11): 1813–21.