Recombinant Rabbit Monoclonal Anti- VEGFA antibody
Catalog#BRM1118-2
Lot # Check on the product label
Size 100 μl
Isotype IgG
Host Rabbit
Reactivity
Human, mouse
Immunogen A synthetic peptide of human VEGFA.
Clone ID 3E2
Synonyms MVCD1, Vascular endothelial growth factor A, vascular endothelial growth factor, Vascular permeability factor, VEGF120, VPF
Content Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.
Recommend Application
Immunohistochemistry,IHC-P(1:100)
Immunocytochemistry, ICC/IF (1:200-500)
Flow Cytometry, Flow-Cyt (1:20)
Other applications have not been tested.
The optimal dilutions should be determined by end user.
Storage Instruction
Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.
Avoid repeated freeze and thaw cycles.
Background
Vascular endothelial growth factor (VEGF) is encoded by VEGF gene, which contains 8 exons and maps to 6p21.3. It is a signal protein produced by cells that stimulates vasculogenesis and angiogenesis. Millauer et al. (1994) observed in mouse that its expression was upregulated by hypoxia and that its cell surface receptor, Flk1, is exclusively expressed in endothelial cells. Overexpression of VEGF can cause vascular disease in the retina of the eye and other parts of the body. Drugs such as bevacizumab can inhibit VEGF and control or slow those diseases. The VEGF is a candidate hormone for facilitating glucose passage across the blood-brain barrier under critical conditions.
Reference
1. Vincenti, V., Cassano, C., Rocchi, M., Persico, M. G. Assignment of the vascular endothelial growth factor gene to human chromosome 6p21.3. Circulation 93: 1493-1495, 1996.
2. Millauer, B., Shawver, L. K., Plate, K. H., Risau, W., Ullrich, A. Glioblastoma growth inhibited in vivo by a dominant-negative Flk-1 mutant. Nature 367: 576-579, 1994.
3. Dantz, D., Bewersdorf, J., Fruehwald-Schultes, B., Kern, W., Jelkmann, W., Born, J., Fehm, H. L., Peters, A. Vascular endothelial growth factor: a novel endocrine defensive response to hypoglycemia. J. Clin. Endocr. Metab. 87: 835-840, 2002
