Recombinant Rabbit Monoclonal Anti- PHD1 antibody
|
Catalog# |
BRM1119-2 |
|---|---|
|
Lot # |
Check on the product label |
|
Size |
50 μl |
| Isotype |
IgG |
| Host | Rabbit |
|
Reactivity |
Human, mouse, rat |
| Immunogen | A synthetic peptide of human PHD1. |
|
Clone ID |
10L4 |
| Synonyms | EGLN2; EIT6; HIF P4H 1; HIF PH1; HIF prolyl hydroxylase 1; HIF-PH1; HIF-prolyl hydroxylase 1; HIFPH 1; HIFPH1; HPH 3; HPH-1 |
| Content | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Recommend Application |
Western Blot, WB (1:1000-5000) The optimal dilutions should be determined by end user. |
| Storage Instruction | Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year. Avoid repeated freeze and thaw cycles. |
|
Background |
Prolyl hydroxylase domain proteins HIF PHD1, HIF PHD2 and HIF PHD3 (known as PHD1, PHD2 and PHD3 in rodents, respectively) can hydroxylate HIF-α subunits. Hypoxia-inducible factor (HIF) is a transcriptional regulator important in several aspects of oxygen homeostasis. The prolyl hydroxylases catalyze the posttranslational formation of 4-hydroxyproline in HIF-α proteins. HIF PHD1, which is widely expressed, with highest levels of expression in testis, functions as a cellular oxygen sensor and is important in cell growth regulation. HIF PHD1 can localize to the nucleus or the cytoplasm and is also detected in hormone responsive tissues, such as normal and cancerous mammary, ovarian and prostate epithelium. HIF PHD1 is encoded by EGLN2, which maps to chromosome 19q13.3. HIF PHD2 is regarded as the main cellular oxygen sensor, as RNA interference against HIF PHD2, but not HIF PHD1 or HIF PHD3, is enough to stabilize HIF-1α in normoxia. HIF PHD2, a direct HIF target gene, is expressed mainly in skeletal muscle, heart, kidney and brain. |
|
Reference |
1. Lalevée S et al. miR455 is linked to hypoxia signaling and is deregulated in preeclampsia. Cell Death Dis 5:e1408 (2014). 2. Van Welden S et al. Differential expression of prolyl hydroxylase 1 in patients with ulcerative colitis versus patients with Crohn\'s disease/infectious colitis and healthy controls. J Inflamm (Lond) 10:36 (2013) |
