Recombinant Rabbit Monoclonal Anti- TPX2 antibody

Catalog# BRM1120-2

Lot # Check on the product label

Size 100 μl

Isotype IgG

Host Rabbit

Reactivity 

Human, mouse, rat

Immunogen Recombinant protein of human TPX2.

Clone ID  6F6

Synonyms C20orf1, C20orf2, DIL-2, DIL2, FLS353, GD:C20orf1, HCA519, HCTP4, REPP86, p100, microtubule nucleation factor, TPX2 microtubule nucleation factor

Content Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.

Recommend Application

Western Blot, WB (1:1000)

Immunohistochemistry,IHC-P(1:200-1000)  

Flow Cytometry, Flow-Cyt (1:20-1:50)

Immunocytochemistry, ICC/IF (1:50)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.

Avoid repeated freeze and thaw cycles.

Background

Targeting protein for Xklp2 is a protein that in humans is encoded by the TPX2 gene. It is one of the many spindle assembly factors that play a key role in inducing microtubule assembly and growth during M phase. TPX2 has been reported to have two NLS-containing domains that mediate its localization to microtubules; one in the amino-terminal domain, and the other in the carboxy-terminal domain. In addition to an NLS, the carboxy-terminal domain of TPX2 consists of tandem repeats that cover over two-thirds of the protein and are computationally predicted to consist of predominantly alpha-helical content.

Reference

1. Sanchez-Pulido L, Perez L, Kuhn S, Vernos I, Andrade-Navarro MA (October 2016). "The C-terminal domain of TPX2 is made of alpha-helical tandem repeats". BMC Structural Biology. 16 (1): 17.

2. Alfaro-Aco R, Thawani A, Petry S (April 2017). "Structural analysis of the role of TPX2 in branching microtubule nucleation". The Journal of Cell Biology. 216 (4): 983–997.