Recombinant Rabbit Monoclonal Anti- DGKZ antibody

Catalog#  BRM1121-1

Lot # Check on the product label

Size 50 μl

Isotype IgG

Host Rabbit

Reactivity 

Human, mouse, rat

Immunogen Recombinant protein of human DGKZ/DGK-zeta.

Clone ID  6X2

Synonyms DAGK5, DAGK6, DGK-ZETA, hDGKzeta, diacylglycerol kinase zeta

Content Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.

Recommend Application

Western Blot, WB (1:1000)

Immunohistochemistry,IHC-P(1:500-2000)  

Flow Cytometry, Flow-Cyt (1:200-1:500)

Immunocytochemistry, ICC/IF (1:50)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.

Avoid repeated freeze and thaw cycles.

Background

Diacylglycerol kinase zeta is an enzyme that in humans is encoded by the DGKZ gene. The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and three transcript variants encoding three distinct isoforms have been identified. DGKZ has been shown to interact with P110α.

Reference

1. Bunting M, Tang W, Zimmerman GA, McIntyre TM, Prescott SM (Jun 1996). "Molecular cloning and characterization of a novel human diacylglycerol kinase zeta". J Biol Chem. 271 (17): 10230–6.

2. "Entrez Gene: DGKZ diacylglycerol kinase, zeta 104kDa".

3. Luo, Bai; Prescott Stephen M; Topham Matthew K (Oct 2003). "Protein kinase C alpha phosphorylates and negatively regulates diacylglycerol kinase zeta". J. Biol. Chem. 278 (41): 39542–7.