Recombinant Rabbit Monoclonal Anti- IRF5 antibody

Catalog#  BRM1122-1

Lot # Check on the product label

Size 50 μl

Isotype IgG

Host Rabbit

Reactivity 

Human, mouse, rat

Immunogen Recombinant protein of human IRF5.

Clone ID  3K1

Synonyms SLEB10; interferon regulatory factor 5

Content Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.

Recommend Application

Western Blot, WB (1:1000)

Immunohistochemistry, IHC-P (1:200-500)
Immunocytochemistry,ICC/IF(1:500-1000)

Immunoprecipitation, IP (1:100-200)

Flow Cytometry, Flow-Cyt (1:20-1:50)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.

Avoid repeated freeze and thaw cycles.

Background

Interferon regulatory factor 5 is a protein that in humans is encoded by the IRF5 gene. The IRF family is a group of transcription factors that are involved in signaling for virus responses in mammals along with regulation of certain cellular functions. IRF5 is a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist. The regulatory and repression regions of the IRF family are mainly located in the C-terminal of the IRF.

Reference

1. Negishi H, Taniguchi T, Yanai H (November 2018). "The Interferon (IFN) Class of Cytokines and the IFN Regulatory Factor (IRF) Transcription Factor Family". Cold Spring Harbor Perspectives in Biology. 10 (11): a028423.

2. Chistiakov DA, Myasoedova VA, Revin VV, Orekhov AN, Bobryshev YV (January 2018). "The impact of interferon-regulatory factors to macrophage differentiation and polarization into M1 and M2". Immunobiology. 223 (1): 101–111.