Recombinant Rabbit Monoclonal Anti- SQSTM1/p62 antibody

Catalog#  BRM1124-1

Lot # Check on the product label

Size 50 μl

Isotype IgG

Host Rabbit

Reactivity 

Human, mouse, rat

Immunogen A synthetic peptide of human SQSTM1/p62.

Clone ID  8G7

Synonyms SQSTM1, A170, OSIL, PDB3; ZIP3, p60, p62, p62B, FTDALS3, Sequestosome 1, NADGP, DMRV

Content Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.

Recommend Application

Western Blot, WB (1:5000-50000)

Immunohistochemistry, IHC-P (1:200-500)
Immunocytochemistry, ICC/IF (1:200-500)

Flow Cytometry, Flow-Cyt (1:20)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.

Avoid repeated freeze and thaw cycles.

Background

Sequestosome-1 is a protein that in humans is encoded by the SQSTM1 gene. Also known as the ubiquitin-binding protein p62, it is an autophagosome cargo protein that targets other proteins that bind to it for selective autophagy. By interacting with GATA4 and targeting it for degradation, it can inhibit GATA-4 associated senescence and senescence-associated secretory phenotype. Model organisms have been used in the study of SQSTM1 function. A conditional knockout mouse line, called Sqstm1tm1a(KOMP)Wtsi was generated as part of the International Knockout Mouse Consortium program-a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.

Reference

1. Joung I, Strominger JL, Shin J (July 1996). "Molecular cloning of a phosphotyrosine-independent ligand of the p56lck SH2 domain". Proc Natl Acad Sci U S A. 93 (12): 5991–5.

2. Devergne O, Hummel M, Koeppen H, Le Beau MM, Nathanson EC, Kieff E, Birkenbach M (February 1996). "A novel interleukin-12 p40-related protein induced by latent Epstein-Barr virus infection in B lymphocytes". J Virol. 70 (2): 1143–53.

3. Cassidy LD, Narita M (2015). "GATA get a hold on senescence" (PDF). Science. 349 (6255): 1448–9