Recombinant Rabbit Monoclonal Anti- SCD1 antibody

Catalog#  BRM1126-1

Lot # Check on the product label

Size 50 μl

Isotype IgG

Host Rabbit

Reactivity 

Human, mouse, rat

Immunogen Recombinant protein of human SCD1.

Clone ID  2W1

Synonyms FADS5; Acyl-CoA desaturase; Delta(9)-desaturase; Stearoyl-CoA desaturase; Fatty acid desaturase

Content Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.

Recommend Application

Western Blot, WB (1:1000)

Immunohistochemistry,IHC-P (1:200-2000)
Immunocytochemistry, ICC/IF (1:100)

Immunoprecipitation, IP (1:20-50)

Flow Cytometry, Flow-Cyt (1:200)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.

Avoid repeated freeze and thaw cycles.

Background

Stearoyl-CoA desaturase (Δ-9-desaturase or SCD-1) is an endoplasmic reticulum enzyme that catalyzes the rate-limiting step in the formation of monounsaturated fatty acids (MUFAs), specifically oleate and palmitoleate from stearoyl-CoA and palmitoyl-CoA. Oleate and palmitoleate are major components of membrane phospholipids, cholesterol esters and alkyl-diacylglycerol. In humans, the enzyme is present in two isoforms, encoded respectively by the SCD1 and SCD5 genes. Stearoyl-CoA desaturase-1 is a key enzyme in fatty acid metabolism. It is responsible for forming a double bond in stearoyl-CoA. This is how the monounsaturated fatty acid oleic acid is produced from the saturated fatty acid, stearic acid.

Reference

1. Paton CM, Ntambi JM (2017-03-08). "Biochemical and physiological function of stearoyl-CoA desaturase". American Journal of Physiology. Endocrinology and Metabolism. 297 (1): E28 – E37.

2. "Entrez Gene: Stearoyl-CoA desaturase (delta-9-desaturase)". Retrieved 2011-09-29.

3. "SCD5 stearoyl-CoA desaturase 5 [Homo sapiens (human) ]". Gene. National Library of Medicine. 5 March 2024. Gene ID No. 79966. Retrieved 22 March 2024.

4. Igal RA, Sinner DI (2021). "Stearoyl-CoA desaturase 5 (SCD5), a Δ-9 fatty acyl desaturase in search of a function". Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1866 (1) 158840.